Kent E. Vrana All publications ( 83 ) Jones JD. Carney ST. Vrana KE. Norford DC. Howlett AC. Cannabinoid receptor-mediated translocation of NO-sensitive guanylyl cyclase and production of cyclic GMP in neuronal cells. 2008 Jan. Neuropharmacology. 54(1):23-30. Freeman WM. Patel KM. Brucklacher RM. Lull ME. Erwin M. Morgan D. Roberts DC. Vrana KE. Persistent Alterations in Mesolimbic Gene Expression with Abstinence from Cocaine Self-Administration. 2007 Sep 12. Neuropsychopharmacology. . Barthelery M. Salli U. Vrana KE. Nuclear Proteomics and Directed Differentiation of Embryonic Stem Cells. 2007 Nov 14. Stem Cells Dev. . Fitzpatrick LR. Hertzog KL. Quatse AL. Koltun WA. Small JS. Vrana K. Effects of the probiotic formulation VSL#3 on colitis in weanling rats. 2007 May. J Pediatr Gastroenterol Nutr. 44(5):561-70. Bowyer JF. Pogge AR. Delongchamp RR. O'Callaghan JP. Patel KM. Vrana KE. Freeman WM. A threshold neurotoxic amphetamine exposure inhibits parietal cortex expression of synaptic plasticity-related genes. 2007 Jan 5. Neuroscience. 144(1):66-76. Ward GR. Franklin SO. Gerald TM. Dempsey KT. Clodfelter DE. Krissinger DJ. Patel KM. Vrana KE. Howlett AC. Glucocorticoids plus opioids up-regulate genes that influence neuronal function. 2007 Aug. Cell Mol Neurobiol. 27(5):651-60. Salli U. Long SW. Carlsen WS. Vrana KE. Stem cell biology should be taught in high schools. 2007. CBE Life Sci Educ. 6(4):283-4. Lull ME. Freeman WM. Myers JL. Midgley F. Kimatian SJ. Undar A. Vrana KE. Plasma proteomics: a noninvasive window on pathology and pediatric cardiac surgery. 2006 Sep-Oct. ASAIO J. 52(5):562-6. Carkaci-Salli N. Flanagan JM. Martz MK. Salli U. Walther DJ. Bader M. Vrana KE. Functional domains of human tryptophan hydroxylase 2 (hTPH2). 2006 Sep 22. J Biol Chem. 281(38):28105-12. National Institute of General Medical Sciences Freeman WM. Gooch RS. Lull ME. Worst TJ. Walker SJ. Xu AS. Green H. Pierre PJ. Grant KA. Vrana KE. Apo-AII is an elevated biomarker of chronic non-human primate ethanol self-administration. 2006 May-Jun. Alcohol Alcohol. 41(3):300-5. National Institute on Alcohol Abuse and Alcoholism National Institute on Drug Abuse Freeman WM. Lull ME. Guilford MT. Vrana KE. Depletion of abundant proteins from non-human primate serum for biomarker studies. 2006 May. Proteomics. 6(10):3109-13. National Institute on Drug Abuse Cibelli JB. Cunniff K. Vrana KE. Embryonic stem cells from parthenotes. 2006. Methods Enzymol. 418:117-35. Worst TJ. Tan JC. Robertson DJ. Freeman WM. Hyytia P. Kiianmaa K. Vrana KE. Transcriptome analysis of frontal cortex in alcohol-preferring and nonpreferring rats. 2005 May 15. J Neurosci Res. 80(4):529-38. Worst TJ. Vrana KE. Alcohol and gene expression in the central nervous system. 2005 Jan-Feb. Alcohol Alcohol. 40(1):63-75. McCracken CB. Hamby SM. Patel KM. Morgan D. Vrana KE. Roberts DC. Extended cocaine self-administration and deprivation produces region-specific and time-dependent changes in connexin36 expression in rat brain. 2005 Dec 1. Synapse. 58(3):141-50. McCracken CB. Patel KM. Vrana KE. Paul DL. Roberts DC. Amphetamine withdrawal produces region-specific and time-dependent changes in connexin36 expression in rat brain. 2005 Apr. Synapse. 56(1):39-44. Stredrick DL. Stokes AH. Worst TJ. Freeman WM. Johnson EA. Lash LH. Aschner M. Vrana KE. Manganese-induced cytotoxicity in dopamine-producing cells. 2004 Jun. Neurotoxicology. 25(4):543-53. Kasinathan C. Vrana K. Beretta L. Thomas P. Gooch R. Worst T. Walker S. Xu A. Pierre P. Green H. Grant K. Manowitz P. The future of proteomics in the study of alcoholism. 2004 Feb. Alcohol Clin Exp Res. 28(2):228-32. Walker SJ. Worst TJ. Freeman WM. Vrana KE. Functional genomic analysis in pain research using hybridization arrays. 2004. Methods Mol Med. 99:239-53. Vrana KE. Hipp JD. Goss AM. McCool BA. Riddle DR. Walker SJ. Wettstein PJ. Studer LP. Tabar V. Cunniff K. Chapman K. Vilner L. West MD. Grant KA. Cibelli JB. Nonhuman primate parthenogenetic stem cells. 2003 Sep 30. Proc Natl Acad Sci U S A. 100 Suppl 1:11911-6. Yohrling GJ. Jiang GC. DeJohn MM. Miller DW. Young AB. Vrana KE. Cha JH. Analysis of cellular, transgenic and human models of Huntington's disease reveals tyrosine hydroxylase alterations and substantia nigra neuropathology. 2003 Nov 6. Brain Res Mol Brain Res. 119(1):28-36. Vrana KE. Freeman WM. Aschner M. Use of microarray technologies in toxicology research. 2003 Jun. Neurotoxicology. 24(3):321-32. National Institute on Drug Abuse Agency for Health Care Policy and Research Patel VB. Chaurand P. Caprioli RM. Austen BM. Frears ER. Manca F. Davies H. Vrana KE. Wheeler M. Preedy VR. Emerging techniques in biomedical research and their application to alcohol toxicity. 2003 Feb. Alcohol Clin Exp Res. 27(2):348-53. Walker SJ. Worst TJ. Vrana KE. Semiquantitative real-time PCR for analysis of mRNA levels. 2003. Methods Mol Med. 79:211-27. Worst TJ. Freeman WM. Walker SJ. Vrana KE. Systematic screening of gene expression using a cDNA macroarray. 2003. Methods Mol Med. 79:243-59. National Institute on Alcohol Abuse and Alcoholism National Institute on Drug Abuse Yohrling IV GJ. Jiang GC. DeJohn MM. Robertson DJ. Vrana KE. Cha JH. Inhibition of tryptophan hydroxylase activity and decreased 5-HT1A receptor binding in a mouse model of Huntington's disease. 2002 Sep. J Neurochem. 82(6):1416-23. National Institute of General Medical Sciences National Institute of Neurological Disorders and Stroke Freeman WM. Brebner K. Patel KM. Lynch WJ. Roberts DC. Vrana KE. Repeated cocaine self-administration causes multiple changes in rat frontal cortex gene expression. 2002 Oct. Neurochem Res. 27(10):1181-92. National Institute on Drug Abuse Haycock JW. Kumer SC. Lewis DA. Vrana KE. Stockmeier CA. A monoclonal antibody to tryptophan hydroxylase: applications and identification of the epitope. 2002 Mar 15. J Neurosci Methods. 114(2):205-12. National Institute of Mental Health National Institute of General Medical Sciences National Institute of Neurological Disorders and Stroke National Institute on Drug Abuse Freeman WM. Dougherty KE. Vacca SE. Vrana KE. An interactive database of cocaine-responsive gene expression. 2002 Mar 12. ScientificWorldJournal. 2:701-6. Freeman WM. Brebner K. Lynch WJ. Patel KM. Robertson DJ. Roberts DC. Vrana KE. Changes in rat frontal cortex gene expression following chronic cocaine. 2002 Jul 15. Brain Res Mol Brain Res. 104(1):11-20. National Institute on Drug Abuse Weber S. Dorman DC. Lash LH. Erikson K. Vrana KE. Aschner M. Effects of manganese (Mn) on the developing rat brain: oxidative-stress related endpoints. 2002 Jul. Neurotoxicology. 23(2):169-75. National Institute of Environmental Health Sciences Cibelli JB. Grant KA. Chapman KB. Cunniff K. Worst T. Green HL. Walker SJ. Gutin PH. Vilner L. Tabar V. Dominko T. Kane J. Wettstein PJ. Lanza RP. Studer L. Vrana KE. West MD. Parthenogenetic stem cells in nonhuman primates. 2002 Feb 1. Science. 295(5556):819. National Institute on Alcohol Abuse and Alcoholism Stokes AH. Freeman WM. Mitchell SG. Burnette TA. Hellmann GM. Vrana KE. Induction of GADD45 and GADD153 in neuroblastoma cells by dopamine-induced toxicity. 2002 Dec. Neurotoxicology. 23(6):675-84. National Institute on Drug Abuse National Institute of General Medical Sciences Blanchard-Fillion B. Souza JM. Friel T. Jiang GC. Vrana K. Sharov V. Barrón L. Schöneich C. Quijano C. Alvarez B. Radi R. Przedborski S. Fernando GS. Horwitz J. Ischiropoulos H. Nitration and inactivation of tyrosine hydroxylase by peroxynitrite. 2001 Dec 7. J Biol Chem. 276(49):46017-23. Walker SJ. Grant KA. Vrana KE. Examination of a CYP2E1 repeat polymorphism in a monkey model of alcohol abuse. 2001 Aug. Alcohol Clin Exp Res. 25(8):1114-8. National Institute on Alcohol Abuse and Alcoholism Freeman WM. Nader MA. Nader SH. Robertson DJ. Gioia L. Mitchell SM. Daunais JB. Porrino LJ. Friedman DP. Vrana KE. Chronic cocaine-mediated changes in non-human primate nucleus accumbens gene expression. 2001 Apr. J Neurochem. 77(2):542-9. National Institute on Drug Abuse Freeman WM. Brebner K. Lynch WJ. Robertson DJ. Roberts DC. Vrana KE. Cocaine-responsive gene expression changes in rat hippocampus. 2001. Neuroscience. 108(3):371-80. National Institute on Drug Abuse Jiang GC. Yohrling GJ. Schmitt JD. Vrana KE. Schmitt IV. Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling. 2000 Sep 29. J Mol Biol. 302(4):1005-17. National Institute of General Medical Sciences Freeman WM. Robertson DJ. Vrana KE. Fundamentals of DNA hybridization arrays for gene expression analysis. 2000 Nov. Biotechniques. 29(5):1042-6, 1048-55. National Institute on Alcohol Abuse and Alcoholism National Institute on Drug Abuse Stokes AH. Xu Y. Daunais JA. Tamir H. Gershon MD. Butkerait P. Kayser B. Altman J. Beck W. Vrana KE. p-ethynylphenylalanine: a potent inhibitor of tryptophan hydroxylase. 2000 May. J Neurochem. 74(5):2067-73. National Institute of Diabetes and Digestive and Kidney Diseases National Institute of General Medical Sciences National Institute on Drug Abuse Stokes AH. Lewis DY. Lash LH. Jerome WG. Grant KW. Aschner M. Vrana KE. Dopamine toxicity in neuroblastoma cells: role of glutathione depletion by L-BSO and apoptosis. 2000 Mar 6. Brain Res. 858(1):1-8. National Institute of General Medical Sciences National Institute on Drug Abuse Freeman WM. Yohrling GJ. Daunais JB. Gioia L. Hart SL. Porrino LJ. Davies HM. Vrana KE. A cocaine analog, 2beta-propanoyl-3beta-(4-tolyl)-tropane (PTT), reduces tyrosine hydroxylase in the mesolimbic dopamine pathway. 2000 Dec 22. Drug Alcohol Depend. 61(1):15-21. National Institute on Drug Abuse Yohrling GJ. Jiang GC. Mockus SM. Vrana KE. Intersubunit binding domains within tyrosine hydroxylase and tryptophan hydroxylase. 2000 Aug 1. J Neurosci Res. 61(3):313-20. National Institute of General Medical Sciences National Institute on Alcohol Abuse and Alcoholism Letchworth SR. Sexton T. Childers SR. Vrana KE. Vaughan RA. Davies HM. Porrino LJ. Regulation of rat dopamine transporter mRNA and protein by chronic cocaine administration. 1999 Nov. J Neurochem. 73(5):1982-9. National Institute on Drug Abuse Vrana KE. How the regulatory and catalytic domains get together. 1999 May. Nat Struct Biol. 6(5):401-2. Stokes AH. Hastings TG. Vrana KE. Cytotoxic and genotoxic potential of dopamine. 1999 Mar 15. J Neurosci Res. 55(6):659-65. National Institute on Drug Abuse National Institute of Neurological Disorders and Stroke Freeman WM. Walker SJ. Vrana KE. Quantitative RT-PCR: pitfalls and potential. 1999 Jan. Biotechniques. 26(1):112-22, 124-5. National Institute on Drug Abuse Yohrling GJ. Mockus SM. Vrana KE. Identification of amino-terminal sequences contributing to tryptophan hydroxylase tetramer formation. 1999 Feb. J Mol Neurosci. 12(1):23-34. National Institute of General Medical Sciences National Institute on Alcohol Abuse and Alcoholism Aschner M. Vrana KE. Zheng W. Manganese uptake and distribution in the central nervous system (CNS). 1999 Apr-Jun. Neurotoxicology. 20(2-3):173-80. National Institute of Environmental Health Sciences Vogt BA. Vogt LJ. Vrana KE. Gioia L. Meadows RS. Challa VR. Hof PR. Van Hoesen GW. Multivariate analysis of laminar patterns of neurodegeneration in posterior cingulate cortex in Alzheimer's disease. 1998 Sep. Exp Neurol. 153(1):8-22. Mockus SM. Vrana KE. Advances in the molecular characterization of tryptophan hydroxylase. 1998 Jun. J Mol Neurosci. 10(3):163-79. National Institute of General Medical Sciences National Institute on Alcohol Abuse and Alcoholism Mockus SM. Yohrling GJ. Vrana KE. Tyrosine hydroxylase and tryptophan hydroxylase do not form heterotetramers. 1998 Feb. J Mol Neurosci. 10(1):45-51. National Institute of General Medical Sciences National Institute on Alcohol Abuse and Alcoholism Xu Y. Stokes AH. Roskoski R. Vrana KE. Dopamine, in the presence of tyrosinase, covalently modifies and inactivates tyrosine hydroxylase. 1998 Dec 1. J Neurosci Res. 54(5):691-7. National Institute of General Medical Sciences National Institute of Neurological Disorders and Stroke Gioia L. Vogt LJ. Freeman WM. Flood A. Vogt BA. Vrana KE. PCR-based apolipoprotein E genotype analysis from archival fixed brain. 1998 Apr 30. J Neurosci Methods. 80(2):209-14. National Institute on Aging National Institute on Drug Abuse National Institute of Mental Health Aschner M. Stark S. Vrana KE. Conklin DR. Acidosis-induced metallothionein (MT) mRNA expression in neonatal rat primary astrocyte cultures. 1998 Apr. Neurotoxicology. 19(2):227-36. National Institute of Environmental Health Sciences Mockus SM. Kumer SC. Vrana KE. Carboxyl terminal deletion analysis of tryptophan hydroxylase. 1997 Oct 17. Biochim Biophys Acta. 1342(2):132-40. National Institute of General Medical Sciences National Institute on Alcohol Abuse and Alcoholism National Institute on Drug Abuse Kumer SC. Mockus SM. Rucker PJ. Vrana KE. Amino-terminal analysis of tryptophan hydroxylase: protein kinase phosphorylation occurs at serine-58. 1997 Oct. J Neurochem. 69(4):1738-45. National Institute of General Medical Sciences National Institute on Alcohol Abuse and Alcoholism National Institute on Drug Abuse van de Pavert SA. Clarke IJ. Rao A. Vrana KE. Schwartz J. Effects of vasopressin and elimination of corticotropin-releasing hormone-target cells on pro-opiomelanocortin mRNA levels and adrenocorticotropin secretion in ovine anterior pituitary cells. 1997 Jul. J Endocrinol. 154(1):139-47. National Institute on Alcohol Abuse and Alcoholism Mockus SM. Kumer SC. Vrana KE. A chimeric tyrosine/tryptophan hydroxylase. The tyrosine hydroxylase regulatory domain serves to stabilize enzyme activity. 1997 Aug. J Mol Neurosci. 9(1):35-48. National Institute of General Medical Sciences National Institute on Alcohol Abuse and Alcoholism National Institute on Drug Abuse Xu Y. Stokes AH. Freeman WM. Kumer SC. Vogt BA. Vrana KE. Tyrosinase mRNA is expressed in human substantia nigra. 1997 Apr. Brain Res Mol Brain Res. 45(1):159-62. National Institute on Aging National Institute of General Medical Sciences National Institute on Drug Abuse Stokes AH. Brown BG. Lee CK. Doolittle DJ. Vrana KE. Tyrosinase enhances the covalent modification of DNA by dopamine. 1996 Nov. Brain Res Mol Brain Res. 42(1):167-70. National Institute of General Medical Sciences Vrana KE. Advancing technologies in gene amplification. 1996 Nov. Trends Biotechnol. 14(11):413-5. Freeman WM. Vrana SL. Vrana KE. Use of elevated reverse transcription reaction temperatures in RT-PCR. 1996 May. Biotechniques. 20(5):782-3. National Institute on Drug Abuse Kumer SC. Vrana KE. Intricate regulation of tyrosine hydroxylase activity and gene expression. 1996 Aug. J Neurochem. 67(2):443-62. National Institute on Drug Abuse National Institute of General Medical Sciences Kluttz BW. Vrana KE. Dworkin SI. Childers SR. Effects of morphine on forskolin-stimulated pro-enkephalin mRNA levels in rat striatum: a model for acute and chronic opioid actions in brain. 1995 Sep. Brain Res Mol Brain Res. 32(2):313-20. National Institute on Drug Abuse Dopke KL. Vrana KE. Vogt LJ. Vogt BA. AF-DX 384 binding in rabbit cingulate cortex: two site kinetics and section autoradiography. 1995 Jul. J Pharmacol Exp Ther. 274(1):562-9. National Institute on Aging Vrana SL. Kluttz BW. Vrana KE. Application of quantitative RT-PCR to the analysis of dopamine receptor mRNA levels in rat striatum. 1995 Dec 1. Brain Res Mol Brain Res. 34(1):127-34. National Institute on Drug Abuse Walker SJ. Liu X. Roskoski R. Vrana KE. Catalytic core of rat tyrosine hydroxylase: terminal deletion analysis of bacterially expressed enzyme. 1994 May 18. Biochim Biophys Acta. 1206(1):113-9. National Institute of General Medical Sciences National Institute of Neurological Disorders and Stroke Vrana KE. Walker SJ. Rucker P. Liu X. A carboxyl terminal leucine zipper is required for tyrosine hydroxylase tetramer formation. 1994 Dec. J Neurochem. 63(6):2014-20. National Institute of General Medical Sciences Vrana KE. Rucker PJ. Kumer SC. Recombinant rabbit tryptophan hydroxylase is a substrate for cAMP-dependent protein kinase. 1994. Life Sci. 55(13):1045-52. National Institute of General Medical Sciences Walker SJ. Vrana KE. Pituitary corticotroph function during the stress hyporesponsive period in neonatal rats. 1993 Jun. Neuroendocrinology. 57(6):1003-10. National Center for Research Resources Vrana SL. Dworkin SI. Vrana KE. Radioenzymatic assay for tryptophan hydroxylase: [3H]H2O release assessed by charcoal adsorption. 1993 Jun. J Neurosci Methods. 48(1-2):123-9. National Institute of General Medical Sciences National Institute on Drug Abuse Vrana SL. Vrana KE. Koves TR. Smith JE. Dworkin SI. Chronic cocaine administration increases CNS tyrosine hydroxylase enzyme activity and mRNA levels and tryptophan hydroxylase enzyme activity levels. 1993 Dec. J Neurochem. 61(6):2262-8. National Institute on Drug Abuse National Institute of General Medical Sciences Vrana SL. Azzaro AJ. Vrana KE. Chronic selegiline administration transiently decreases tyrosine hydroxylase activity and mRNA in the rat nigrostriatal pathway. 1992 May. Mol Pharmacol. 41(5):839-44. Public Health Service Lamport-Vrana SJ. Vrana KE. Fedan JS. Involvement of ecto-phosphoryl transfer in contractions of the smooth muscle of the guinea pig vas deferens to adenosine 5'-triphosphate. 1991 Jul 1. J Pharmacol Exp Ther. 258(1):339-48. National Institute of General Medical Sciences Roskoski R. Wilgus H. Vrana KE. Inactivation of tyrosine hydroxylase by pterin substrates following phosphorylation by cyclic AMP-dependent protein kinase. 1990 Oct. Mol Pharmacol. 38(4):541-6. National Institute of Neurological Disorders and Stroke Liu X. Giza CC. Vrana KE. High efficiency transient expression of eukaryotic genes: use of an HSV-1 immediate early promoter (ICP4). 1990 Aug. Biotechniques. 9(2):168-73. National Center for Research Resources Ardekani AM. Walker SJ. Donohue SJ. Stitzel RE. Connors JM. Vrana KE. Adrenocorticotropin and corticosterone levels in pre-weanling spontaneously hypertensive rats. 1989. Life Sci. 44(14):919-25. National Center for Research Resources National Heart, Lung, and Blood Institute National Institute of General Medical Sciences Vrana KE. Churchill ME. Tullius TD. Brown DD. Mapping functional regions of transcription factor TFIIIA. 1988 Apr. Mol Cell Biol. 8(4):1684-96. Kubinec J. Vrana KE. Roskoski R. Paraoxon-induced decrease in the muscarinic acetylcholine receptor content in rat heart. 1987 Apr 29. Eur J Pharmacol. 136(3):295-301. National Heart, Lung, and Blood Institute Vrana KE. Roskoski R. Tyrosine hydroxylase inactivation following cAMP-dependent phosphorylation activation. 1983 Jun. J Neurochem. 40(6):1692-700. National Institute of Neurological Disorders and Stroke National Heart, Lung, and Blood Institute Cook PF. Neville ME. Vrana KE. Hartl FT. Roskoski R. Adenosine cyclic 3',5'-monophosphate dependent protein kinase: kinetic mechanism for the bovine skeletal muscle catalytic subunit. 1982 Nov 9. Biochemistry. 21(23):5794-9. National Heart, Lung, and Blood Institute Vrana KE. Allhiser CL. Roskoski R. Tyrosine hydroxylase activation and inactivation by protein phosphorylation conditions. 1981 Jan. J Neurochem. 36(1):92-100. National Aeronautics and Space Agency